Stable pluripotent stem cell production
September 13, 2014 7:05 PM Subscribe
"Scientists reset human stem cells to earliest developmental state" The paper, published in Cell, is available under a CC-3.0 license. [See: earlier Mefi post]
"The process of generating stem cells in the lab is much easier to control in mouse cells, which can be frozen in a state of naïve pluripotency using a protein called LIF. Human cells are not as responsive to LIF, so they must be controlled in a different way that involves switching key genes on and off. For this reason scientists have been unable to generate human pluripotent cells that are as primitive or as consistent as mouse embryonic stem cells.No question, it's still very very early days on this.
The researchers overcame this problem by introducing two genes – NANOG and KLF2 – causing the network of genes that control the cell to reboot and induce the naïve pluripotent state. Importantly, the introduced genes only need to be present for a short time. Then, like other stem cells, reset cells can self-renew indefinitely to produce large numbers, are stable and can differentiate into other cell types, including nerve and heart cells. - See more at: http://www.cam.ac.uk/research/news/scientists-reset-human-stem-cells-to-earliest-developmental-state."
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