The mayor told the news conference at City Hall: "A week ago I said it was not my intention to apologise to the Daily Mail group or the journalist."
He said that after a week of reflection he had "decided to stand by that position".
"There will be therefore no apology or expression of regret to the Daily Mail Group."
He added: "To the Daily Mail group, no-one in Britain is less qualified to complain about anti-Semitism.
"In truth, those papers were the leading advocate of anti-Semitism in the country for half a century."
He said that while it is true the Daily Mail has moved on from anti-Semitism, it now targets asylum seekers and Muslims.
"For the Mail group the victims may change but the intolerance, hatred and fear pervade every issue of the papers," Mr Livingstone said.
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