I wonder if the experiment was properly blinded to prevent the experimenters from unconsciously influencing the offspring.
All behavior was performed in a double-blind manner and data acquired using automated computer software programs. We are grateful to S. Banerjee, R. Andero-Gali, D. Choi, J. Goodman and F. Morrison for help with ensuring double-blindness of data acquisition and analysis.
Remember, "statistically significant" just means we get a p-value below a threshold that we feel comfortable rejecting the null hypothesis. In this study. We're not getting a meta-analysis of all the other experiments where mice were abused in smelly environments and the results did not reach significance.
"The statistical tests in the paper, both for the behavioral measurements as well as for the size of the M71 glomeruli , use as n, number of samples, the number of F1 and F2 individuals. This would be fine if the individuals were actually independent samples. However, they arise from a presumably small number of FO males. The numbers of FO males are not given in the paper. This is a major concern given that there is a lot of variability in the levels of expression of olfactory receptors in these mice that might be inheritable. As an example, for Figure 1a, the authors compared 16 F1-Ace-C57 mice with 13 F1-Home-C57 mice and find a p value of 0.043, with 27 degrees of freedom . But these 29 mice could have been originated from a very small number of FO mice. The actual n that should be used for the statistics in the whole paper are not the individual number of F1 or F2, but it should be the number of founding F0, for both groups. So the actual p values are larger than reported. Without the information about the size of the F0 populations used in each figure panel, it is hard to interpret the results."
"An excellent point and discussed in Lazic SE, 2013 and references therein.
Also, it is not clear why the dorsal and medial glomerulus have different n for what appears to be same animals. These discrepancies occur for all histological data (e.g. Fig4 panel (i) has n=23 and n=16 for dorsal while panel (j) has n=16 and n=19 for ventral)."
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